Death of Tonsillar B Cells by NETosis

Abstract Regulating B cell death is essential for generating antibodies and maintaining immune tolerance. cells can die by apoptosis, we report that human tonsil cells, but not peripheral blood also NETosis. This density-dependent, characterized the loss of nuclear membrane integrity, release reactive oxygen species, chromatin decondensation. Tonsil secrete high levels TNF, inhibiting TNF prevented By in situfluorescence microscopy, NETosis, as identified hyper citrullination Histone 3, was localized to light zone (LZ) germinal centers normal overlapped with marker CD19/IgM. We propose a model which stimulation LZ induces driven part TNF. provide evidence NETosis may be inhibited an unidentified factor tonsil. The results describe previously form suggest new mechanism maintain homeostasis during responses.